Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.

@article{Heath1968PhotoperoxidationII,
  title={Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.},
  author={R. L. Heath and Lester Packer},
  journal={Archives of biochemistry and biophysics},
  year={1968},
  volume={125 1},
  pages={
          189-98
        }
}
Abstract A photo-induced cyclic peroxidation in isolated chloroplasts is described. In an osmotic buffered medium, chloroplasts upon illumination produce malondialdehyde (MDA)—a decomposition product of tri-unsaturated fatty acid hydroperoxides—bleach endogenous chlorophyll, and consume oxygen. These processes show ( a ) no reaction in the absence of illumination; ( b ) an initial lag phase upon illumination of 10–20 minutes duration; ( c ) a linear phase in which the rate is proportional to… Expand
Photoperoxidation in isolated chloroplasts. II. Role of electron transfer.
TLDR
The results suggest that at high light intensities, overloading of the chlorophyll collection system results in energy being channeled into destructive cyclic peroxidation processes involving the membrane fatty acids. Expand
Activity of Photosystem 2, Lipid Peroxidation, and the Enzymatic Antioxidant Protective System in Heat Shocked Barley Seedlings
The impact of heat shock on minimising the activity of photosystem 2 (PS2) initiating high lipid peroxidation (POL) level and consequently changes in the enzymatic-antioxidant protective system wasExpand
Photosensitized inhibitor formation in isolated, aging chloroplasts
TLDR
The results warrant the conclusion that the blue-light inhibition is closely linked to fatty-acid breakdown which is sensitized by light absorbed by pigments detached from the photosynthetic centers during the process of chloroplast aging in vitro. Expand
Photosynthetic apparatus of chilling-sensitive plants. X. Relationship between superoxide dismutase activity and photoperoxidation of chloroplast lipids☆
TLDR
It is concluded that inactivation of chloroplast cyanide-sensitive superoxide dismutase due to cold and dark treatment of leaves, rather than diminished electron transport, is responsible for accelerated chloroplasts' lipid photoperoxidation. Expand
Photooxidative damage to the cyanobacterium Spirulina platensis mediated by singlet oxygen
Experiments on the specific growth rate, bleaching of pigments, O2 evolution, lipid peroxidation, and loss of sulfhydryl (-SH) content in response to the varying light intensities (2–28 W/m2)Expand
Photodynamic damage to chloroplast membranes, photosensitized oxidation of chloroplast acyl lipid
TLDR
Results provide further evidence for the involvement of 1O2 in chloroplast lipid photooxidative processes. Expand
Light-dependent consumption of oxygen by Vicia faba chloroplasts.
TLDR
Suspensions of Vicia faba chloroplasts exhibit a light-dependent O2 uptake instead of an evolution, and it is suggested that some endogenous components become reduced during illumination and then act as hydrogen donors for a peroxidase reaction mediated by the chloroplast catalase system. Expand
Transient light effects in the Hill reaction of disintegrating chloroplasts in vitro
TLDR
The transient color sensitivity observed earlier in the Hill reaction of disintegrating chloroplasts (red-blue effect) was studied in detail and it is assumed that initially the transfer of energy from blue absorbing accessory pigments to chlorophyll is interrupted and that the same kind of pigment separation happens a second time, some-what later, among thechlorophyll pigments. Expand
Stress ethylene does not originate directly from lipid peroxidation during chilling-enhanced photooxidation
Summary Stress ethylene synthesis was monitored in detached cucumber ( Cucumis sativus ) leaves under conditions (5 °C and 1000μtEinstein m −2 s −1 ) that promote lipid peroxidation. Previous workExpand
Photosynthetic apparatus in chilling-sensitive plants
TLDR
Results indicate that the inhibition of Hill reaction activity due to the cold and dark storage of leaves is caused by both inhibitory FFA released from chloroplast lipids as well as by damage to the thylakoid structure affecting the electron transport within photosystem II. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 21 REFERENCES
PHOTOXIDATION PROCESSES IN PLANTS
TLDR
The rate of photoxidation rises slower than linearly with light intensity; its dependence upon oxygen pressure has the character of a saturation curve. Expand
LIGHT-INDUCED VOLUME CHANGES IN SPINACH CHLOROPLASTS
TLDR
A light-dependent mechanism that results in a slow, high-amplitude swelling of spinach chloroplasts in vitro has been discovered and the existence of an osmotic mechanism for chloroplast swelling in the dark is confirmed. Expand
The mechanism of the photochemical activity of isolated chloroplasts. I. Effect of temperature.
TLDR
The loss of Hill-reaction reactivity of isolated chloroplast fragments is due to at least two different processes: one, significant at temperatures greater than 30 °C. Expand
ADP-ACTIVATED LIPID PEROXIDATION COUPLED TO THE TPNH OXIDASE SYSTEM OF MICROSOMES.
TLDR
The occurrence of a TPNH-induced peroxidation of lipids in rat-liver microsomes is reported and the reaction requires activation by ADP or other pyrophosphates, and appears to be coupled to the TPNh oxidase system of the microsome. Expand
STUDIES ON THE MECHANISM OF ASCORBATE-INDUCED SWELLING AND LYSIS OF ISOLATED LIVER MITOCHANDRIA.
Earlier work (2) demonstrated that low, but not high, concentrations of ascorbate produce a swelling or lytic change in dilute suspensions of isolated liver mit.ochondria. This structural changeExpand
Photochemical oxidation of fatty acid esters with and without chlorophyll
Summary1.It has been confirmed that the principal products formed in the oxidation of methyl oleate by oxygen under a variety of conditions are predominantlytrans hydroperoxides. However no inversionExpand
Atmospheric Oxidation and Antioxidants
Part 1. Autoxidation and Antioxidants - Historical Perspective, G. Scott: The Ageing of Rubber Hydroperoxides and the Autoxidation Chain Reaction Antioxidants Synergism The Effect of the ReactionExpand
On the analysis of photosynthesis by flashlight techniques.
TLDR
The analysis of the primary process using flash photometry on normal plant cells and chloroplasts and on those which have been chemically or physically modified has led to the formulation of a reaction scheme for the transport of electrons in photosynthesis. Expand
Reactivity of Malonaldehyde with Food Constituents
SUMMARY Malonaldehyde and/or substances closely resembling it occur in foods as decomposition products of oxidizing polyunsaturated fatty acids. The reaction of malonaldehyde with various foodExpand
The use of thiobarbituric acid as a measure of fat oxidation
SummaryAn evaluation of thiobarbituric acid (TBA) as an agent for the measurement of fat oxidation was made by the application of several empirical procedures to animal and vegetable fats. AnExpand
...
1
2
3
...