Photopatterning enzymes on polymer monoliths in microfluidic devices for steady-state kinetic analysis and spatially separated multi-enzyme reactions.

@article{Logan2007PhotopatterningEO,
  title={Photopatterning enzymes on polymer monoliths in microfluidic devices for steady-state kinetic analysis and spatially separated multi-enzyme reactions.},
  author={Timothy C Logan and Douglas A S Clark and Timothy B. Stachowiak and Frantisek Svec and Jean M. J. Fr{\'e}chet},
  journal={Analytical chemistry},
  year={2007},
  volume={79 17},
  pages={6592-8}
}
A method for photopatterning multiple enzymes on porous polymer monoliths within microfluidic devices has been developed and used to perform spatially separated multienzymatic reactions. To reduce nonspecific adsorption of enzymes on the monolith, its pore surface was modified by grafting poly(ethylene glycol), followed by surface photoactivation and enzyme… CONTINUE READING