Photonic crystal enhanced microscopy for imaging of live cell adhesion.

  title={Photonic crystal enhanced microscopy for imaging of live cell adhesion.},
  author={Weili Chen and Kenneth D Long and Meng Lu and Vikram Chaudhery and Hojeong Yu and Ji Sun Choi and James Polans and Yue Zhuo and Brendan A. C. Harley and Brian T. Cunningham},
  journal={The Analyst},
  volume={138 20},
A form of microscopy that utilizes a photonic crystal biosensor surface as a substrate for cell attachment enables label-free, quantitative, submicron resolution, time-resolved imaging of cell-surface interactions without cytotoxic staining agents or temporally-unstable fluorophores. Other forms of microscopy do not provide this direct measurement of live cell-surface attachment localization and strength that includes unique, dynamic morphological signatures critical to the investigation of… 
Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)
It is demonstrated that cell–surface contacts and focal adhesion formation can be imaged by two orthogonal label-free modalities in PROM simultaneously, providing a general-purpose tool for kinetic, high axial-resolution monitoring of cell interactions with basement membranes.
Planar Photonic Crystal Biosensor for Quantitative Label‐Free Cell Attachment Microscopy
A fabrication approach that combines nanoreplica molding and a horizontal dipping process is used to planarize the surface of the PC biosensor, which effectively eliminates the directionally biased cellular attachment behaviors that are observed on the grating‐structured surface.
Enhanced live cell imaging via photonic crystal enhanced fluorescence microscopy.
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Label-Free Biosensor Imaging on Photonic Crystal Surfaces
We review the development and application of nanostructured photonic crystal surfaces and a hyperspectral reflectance imaging detection instrument which, when used together, represent a new form of
Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM)
We demonstrate label-free imaging of cell attachment upon a photonic-crystal biosensor surface. Newly-implemented PCEM image-analysis software is used to dynamically visualize individual live-cell
Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy
It is demonstrated that strong field enhancement from plasmonic metasurfaces enables the use of MEIRS as a highly informative analytic technique for real-time monitoring of cells and opens the way to a scalable, high-throughput spectroscopic assay for live cells.
Single nanoparticle detection using photonic crystal enhanced microscopy.
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Cell refractive index for cell biology and disease diagnosis: past, present and future.
An overview of cell refractive index models and measurement techniques including microfluidic chip-based techniques for the last 50 years are provided, the applications and significance of cellRefractive index in cell biology, hematology, and pathology are presented, and future research trends in the field are discussed.


Surface plasmon resonance imaging of cells and surface-associated fibronectin
SPRI is a real-time, low-light-level, label-free imaging technique that allows the simultaneous observation and quantification of protein layers and cellular features and is compatible with live cells such that it is possible to monitor cellular modifications to the extracellular matrix in real- time.
Imaging of the cell surface interface using objective coupled widefield surface plasmon microscopy.
Imaging with total internal reflection fluorescence microscopy for the cell biologist
Benefits of using TIRF include the ability to obtain high-contrast images of fluorophores near the plasma membrane, very low background from the bulk of the cell, reduced cellular photodamage and rapid exposure times.
A detection instrument for enhanced-fluorescence and label-free imaging on photonic crystal surfaces.
An optical imaging system capable of exciting surface-bound fluorophores within the resonant evanescent electric field of a photonic crystal surface and gathering fluorescence emission that is directed toward the imaging objective by the photonic crystals is reported on.
Total internal reflection fluorescence microscopy in cell biology.
Imaging single molecules using total internal reflection fluorescence microscopy (TIRFM).
This review gives a detailed description of important considerations for setting up a TIRFM, based on commercially available systems, and review considerations for purification and labeling of proteins.
Application of photonic crystal enhanced fluorescence to cancer biomarker microarrays.
Dose-response characterization of the photonic crystal antibody microarrays shows the capability to detect common cancer biomarkers in the <2 pg/mL concentration range within a mixed sample.
Leaky-mode assisted fluorescence extraction: application to fluorescence enhancement biosensors.
The first enhanced extraction biosensor that allows for over 20-fold enhancement of the fluorescence signal in detection of the cytokine TNF-alpha by a fluorescence sandwich immunoassay is demonstrated.
Colorimetric resonant reflection as a direct biochemical assay technique
  • B. Cunningham, P. Li, B. Lin, J. Pepper
  • Physics, Biology
    Technical Digest. MEMS 2002 IEEE International Conference. Fifteenth IEEE International Conference on Micro Electro Mechanical Systems (Cat. No.02CH37266)
  • 2002