Photon Upmanship: Why Multiphoton Imaging Is More than a Gimmick

@article{Denk1997PhotonUW,
  title={Photon Upmanship: Why Multiphoton Imaging Is More than a Gimmick},
  author={W. Denk and K. Svoboda},
  journal={Neuron},
  year={1997},
  volume={18},
  pages={351-357}
}
The unique niche that light microscopy occupies in biology is based on the ability to perform observations on living tissue at relatively high spatial resolution. This resolution is limited by the wavelength of light and does not rival that of electron microscopy, which is, however, a fundamentally nonvital form of observation. Other vital microscopies, such as MRI, can neither resolve subcellular structures nor provide the exquisite molecular selectivity that allows the detection of even… Expand
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