Multi-Photon Molecular Excitation in Laser-Scanning Microscopy
Conventional fluorescence microscopy can provide submicron spatial resolution of chemical dynamics within living cells, but is frequently limited in sensitivity and spatial resolution by background due to out-offocus and scattered fluorescence.
Two-photon in vivo imaging of cells
- Physics, BiologyPediatric Nephrology
A short history of, and impetus behind, two-photon imaging, its working mechanics, and emerging technologies related to biological multiphoton imaging are provided.
Two-photon excitation microscopy and its applications in neuroscience.
- Biology, PhysicsMethods in molecular biology
Examples of important applications of 2PE for in vivo imaging of neuronal structure and signals are provided and how it can be combined with optogenetics or photolysis of caged molecules to simultaneously probe and control neuronal activity is described.
Multifocal multiphoton microscopy.
- PhysicsOptics letters
A real-time, direct-view multiphoton excitation fluorescence microscope that provides three-dimensional imaging at high resolution using a rotating microlens disk that permits the observation with the eye of high-resolution two-photon images of (live) samples.
Multiphoton microscopy in neuroscience
- Biology, PhysicsSPIE BiOS
Several cases will be presented from a number of different tissues and organisms, where multi-photon excited laser scanning fluorescence microscopy has been an essential experimental tool for discovery of biochemical coincidence detection in synaptic spines and the clarification of the underlying mechanism.
Two-photon microscopy : sequential imaging studies in vivo
Since its first demonstration over a decade ago, twophoton microscopy has been applied to a variety of imaging tasks and has now become the technique of choice for fluorescence microscopy in thick tissue preparations and in live animals.
Two-photon microscopy: shedding light on the chemistry of vision.
This relatively new method of microscopy has been the subject of numerous technical improvements in terms of the optics and indicators used, improvements that should lead to more detailed biochemical characterizations of the eyes of live animals and even to imaging of the human eye in vivo.
Principles of Multiphoton Microscopy
- BiologyNephron Experimental Nephrology
Improvements in multiphoton fluorescence microscopy will involve optimizing objectives for the unique characteristics of multiphotons, improving the speed at which images may be collected and extending the depth to which imaging may be conducted.
SHOWING 1-10 OF 56 REFERENCES
Intravital imaging of green fluorescent protein using two-photon laser-scanning microscopy.
NONLINEAR ABSORPTION EXTENDS CONFOCAL FLUORESCENCE MICROSCOPY INTO THE ULTRA-VIOLET REGIME AND CONFINES THE ILLUMINATION VOLUME
Two-photon excitation in functional biological imaging.
- Biology, ChemistryJournal of biomedical optics
The contribution that the combination of two-photon absorption with laser scanning20 has made to functional biological imaging in recent years is reviewed.
Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy.
- Physics, BiologyProceedings of the National Academy of Sciences of the United States of America
The measured nonlinear excitation spectra and their photophysical characteristics that empower nonlinear laser microscopy for biological imaging are described.
Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy
- BiologyJournal of Neuroscience Methods
Use of confocal imaging in the study of biological structures.
- BiologyApplied optics
This work has developed a high-speed beam scanning confocal imaging system that can be used in conjunction with a conventional microscope, and has examined a variety of biological material using this system, finding that confocal Imaging gives a marked improvement in quality over conventional techniques.
Two-photon laser scanning fluorescence microscopy.
- Physics, ChemistryScience
The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Application of a femtosecond self-sustaining mode-locked Ti:sapphire laser to the field of laser scanning confocal microscopy
Developments in ultrafast Ti:sapphire laser technology can be applied in the investigation of nonlinear optical processes. We describe the application of a self-sustaining femtosecond Ti:sapphire…
Two-photon scanning photochemical microscopy: mapping ligand-gated ion channel distributions.
- BiologyProceedings of the National Academy of Sciences of the United States of America
It is shown that the highly localized liberation of "caged" neurotransmitters by two-photon absorption-mediated photoactivation can be used in conjunction with recording the induced whole-cell current to determine the distribution of ligand-gated ion channels.
Three-dimensional laser scanning two-photon fluorescence confocal microscopy of polymer materials using a new, efficient upconverting fluorophore.
- Physics, ChemistryScanning
The results suggest the possibility of using two-photon confocal microscopy, in conjunction with highly efficient fluorophores, as a tool to study the surface, interface, and fracture in material science applications.