Photometric analysis of antifading reagents for immunofluorescence with laser and conventional illumination sources.

@article{Boeck1985PhotometricAO,
  title={Photometric analysis of antifading reagents for immunofluorescence with laser and conventional illumination sources.},
  author={Guenther Boeck and Martin Hilchenbach and Konrad Schauenstein and Georg Wick},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1985},
  volume={33 7},
  pages={699-705}
}
Bleaching of stained objects is a major problem in immunofluorescence. The prevention of fluorescence fading would allow longer observation times, photographic documentation, fluorometry, and pattern recognition. Fluorescein kinetics and fluorescence intensities (FI) of fluorescein isothiocyanate (FITC) conjugate-stained Sephadex beads were studied with previously described "antibleaching" reagents using an argon laser as the excitation light source. Eight antibleaching reagents were tested… CONTINUE READING
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