Photoinduced single strand breaks and intrastrand cross-links in an oligonucleotide labeled with 5-bromouracil.

@article{Zdrowowicz2014PhotoinducedSS,
  title={Photoinduced single strand breaks and intrastrand cross-links in an oligonucleotide labeled with 5-bromouracil.},
  author={Magdalena Zdrowowicz and Barbara Michalska and Agnieszka Żylicz-Stachula and Janusz Rak},
  journal={The journal of physical chemistry. B},
  year={2014},
  volume={118 19},
  pages={5009-16}
}
5-Bromouracil (BrU) is photoreactive toward near UVB photons and can be introduced into genomic DNA during its biosynthesis in cells. However, PCR seems to be a simpler approach, which can be used to obtain labeled DNA similar to that synthesized within the cell. In the current work, PCR has been employed and optimized in order to substitute all thymines (besides those present in starters) with BrU in the dsDNA fragment of 80 base pairs (bp) in length. The modified oligonucleotide was… CONTINUE READING