Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium

  title={Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium},
  author={Denong Wang and Gregory T. Carroll and Nicholas J. Turro and Jeffrey T Koberstein and Pavol Kov{\'a}{\vc} and R. P. Saksena and Roberto Adamo and Leonore Herzenberg and Leonard A. Herzenberg and Lawrence Steinman},
Using photogenerated glycan arrays, we characterized a large panel of synthetic carbohydrates for their antigenic reactivities with pathogen‐specific antibodies. We discovered that rabbit IgG antibodies elicited by Bacillus anthracis spores specifically recognize a tetrasaccharide chain that decorates the outermost surfaces of the B. anthracis exosporium. Since this sugar moiety is highly specific for the spores of B. anthracis, it appears to be a key biomarker for detection of B. anthracis… 

Glycan surface antigens from Bacillus anthracis as vaccine targets: current status and future perspectives

  • R. Adamo
  • Biology
    Expert review of vaccines
  • 2014
Carohydrate structures found on surfaces of vegetative cells and spores and the tetrasaccharide unit isolated from the exosporium protein BclA were proven immunogenic in an animal model after covalent linkage to carrier protein.

Synthesis and immunochemical evaluation of a non-methylated disaccharide analogue of the anthrax tetrasaccharide.

The synthesis of a 2-O-demethylated-β-D-anthropyranosyl-(1→3)-α-L-rhamnopyranose disaccharide analogue of this tetrasaccharides from a cyclic sulfate intermediate is reported, suggesting that the disacCharide analogue, easily amenable for a synthetic scale-up, could be used in a glycoconjugate antigen formulation.

Synthesis of anthrose lipidic derivative as mimic of B. anthracis BclA glycoprotein for use in ELISA-like binding assays

ABSTRACT The surfaces of Bacillus anthracis endospores expose anthrose-containing oligosaccharides, which have been considered for use as a target for specific detection of the spores. In this

Glycoarrays with engineered phages displaying structurally diverse oligosaccharides enable high‐throughput detection of glycan–protein interactions

A novel glycan array platform based on surface patterning of engineered glycophages that display unique carbohydrate epitopes that are compatible with surface immobilization procedures and that phage‐displayed oligosaccharides retain the ability to be recognized by different glycan‐binding proteins after immobilization.

Sensitive and specific enzyme immunoassays for antigenic trisaccharide from Bacillus anthracis spores.

A straightforward synthesis of an anthrose-containing trisaccharide derived from Bacillus anthracis was achieved and it was demonstrated that the epitope was mainly made up of the methyl group, the butamido group and the hydroxyl at C-3 of the anthrose unit, the other parts of the trisACcharide appearing little involved in the recognition.

Access to antigens related to anthrose using pivotal cyclic sulfite/sulfate intermediates.

A novel entry to anthrose-containing antigens and precursors is described, starting from D(+)-fucose, makes use of intermediates featuring a cyclic sulfite or sulfate function which serves successively as a protecting and a leaving group.

Development of Antibodies against Anthrose Tetrasaccharide for Specific Detection of Bacillus anthracis Spores

The anthrose-containing tetrasaccharide chain seems to be highly specific for B. anthracis, which makes it a key biomarker for the detection of these spores, and could therefore be useful for the Detection of spores in complex samples.

Glycosylation of BclA Glycoprotein from Bacillus cereus and Bacillus anthracis Exosporium Is Domain-specific*

The present study has established that the collagen-like regions of both B. anthracis and B. cereus are similarly substituted by short O-glycans that bear the species-specific deoxyhexose residues anthrose and the newly observed cereose, respectively, and discovered that the C-terminal globular domains of BclA from both species are substituted by polysaccharide-like O-linked glycans whose structures are also species- specific.

Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins

Examples of the different microarray platforms and applications are presented with a view to give the current state-of-the-art and future prospects in this field.



Glycan arrays lead to the discovery of autoimmunogenic activity of SARS-CoV

Using carbohydrate microarrays, it is found that lectin PHA-L (Phaseolus vulgaris L.), which is specific for a defined complex carbohydrate of ASOR, stained the SARS-CoV-infected cells specifically and intensively, and presents immunologic evidence that a carbohydrate structure of SARS -CoV shares antigenic similarity with host glycan complex carbohydrates.

Identification of the Immunodominant Protein and Other Proteins of the Bacillus anthracis Exosporium

Using a large panel of unique antispore monoclonal antibodies, it is demonstrated that BclA is the immunodominant antigen on the B. anthracis spore surface and showed that the B clA protein and not a carbohydrate constituent directs the dominant immune response.

Species-Specific Peptide Ligands for the Detection of Bacillus anthracis Spores

A family of peptides, with the consensus sequence TYPXPXR, that bind selectively to B. anthracis spores are identified and envision that these peptides can be used as sensors in economical and portable B. Anthracis spore detectors that are essentially free of false-positive signals due to other environmental Bacillus spores.

Attenuated Nontoxinogenic and Nonencapsulated Recombinant Bacillus anthracis Spore Vaccines Protect against Anthrax

Results suggest that some B. anthracisspore-associated antigen(s) may contribute in a significant manner to protective immunity and appear to be more efficacious than the vegetative cell vaccine.

A collagen‐like surface glycoprotein is a structural component of the Bacillus anthracis exosporium

The purification and the characterization of an immunodominant protein of the spore surface was described and this collagen‐like surface protein was named BclA (for Bacillus c ollagen‐ l ike protein of anthracis).

Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells

The development of a carbohydrate-based microarray to extend the scope of biomedical research on carbohydrate-mediated molecular recognition and anti-infection responses is described and the potential of carbohydrate microarrays is demonstrated by the discovery of previously undescribed cellular markers, Dex-Ids.

Carbohydrate microarrays : Protein microarrays

This review attempts to summarize recent progress in using carbohydrate microarray technology to uncover the carbohydrate-based biological signals that are recognized by the human and animal immune systems.

Identification of proteins in the exosporium of Bacillus anthracis.

The protein profiles of SDS-PAGE-separated exosporium extracts were similar for all three strains, suggesting that avirulent variants lacking either or both plasmids are realistic models for studying the exospora from spores of B. anthracis.

Novel Oligosaccharide Side Chains of the Collagen-like Region of BclA, the Major Glycoprotein of the Bacillus anthracis Exosporium*

Spores of Bacillus anthracis, the causative agent of anthrax, are enclosed by a prominent loose fitting layer called the exosporium. The exosporium consists of a basal layer and an external hairlike