Photofootprinting in vivo detects transcription-dependent changes in yeast TATA boxes

@article{Selleck1987PhotofootprintingIV,
  title={Photofootprinting in vivo detects transcription-dependent changes in yeast TATA boxes},
  author={Scott B. Selleck and John Majors},
  journal={Nature},
  year={1987},
  volume={325},
  pages={173-177}
}
To elucidate critical steps in the transcription initiation process, we have devised a protocol for obtaining information about DNA structure and DNA–protein interactions at nucleotide level resolution from intact yeast cells. Our procedure combines the ultraviolet light 'footprinting' method developed by Becker and Wang1 with the 'genomic sequencing' technique described by Church and Gilbert2. Using this approach we were able to detect the binding of GAL 4 protein at sites within the upstream… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 18 extracted citations

Similar Papers

Loading similar papers…