Photochemistry of dyes and fluorochromes used in biology and medicine: some physicochemical background and current applications.

  • J K Sugden
  • Published 2004 in
    Biotechnic & histochemistry : official…
An overview of the basic principles of photochemistry is presented to facilitate discussion of fluorescence, quenching and quantum yields. These topics in turn provide the foundation for an account of fluorescence spectroscopy and its application to microscopy. A brief overview of light microscopy and the application of fluorescence microscopy is given. The… (More)