Photochemistry and fluorescence of bacteriorhodopsin excited in its 280-nm absorption band.

Abstract

Photochemical and fluorescence studies are carried out, exciting bacteriorhodopsin (BR) in its 280-nm absorption band. The data indicate that energy transfer takes place, with a quantum yield of 0.7--0.8, from excited tyrosines and tryptophans to the retinyl chromophore. All of the tyrosine and five to six tryptophan residues are completely quenched by the… (More)

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