Photobleaching in two-photon excitation microscopy.

@article{Patterson2000PhotobleachingIT,
  title={Photobleaching in two-photon excitation microscopy.},
  author={George H. Patterson and David W. Piston},
  journal={Biophysical journal},
  year={2000},
  volume={78 4},
  pages={
          2159-62
        }
}
The intensity-squared dependence of two-photon excitation in laser scanning microscopy restricts excitation to the focal plane and leads to decreased photobleaching in thick samples. However, the high photon flux used in these experiments can potentially lead to higher-order photon interactions within the focal volume. The excitation power dependence of the fluorescence intensity and the photobleaching rate of thin fluorescence samples ( approximately 1 microm) were examined under one- and two… Expand
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