Photobiology of Bacteria

  title={Photobiology of Bacteria},
  author={K. J. Hellingwerf and Wim Crielaard and Wouter D. Hoff and Hans C. P. Matthijs and Luuc R. Mur and B. J. van Rotterdam},
  journal={Antonie van Leeuwenhoek},
The field of photobiology is concerned with the interactions between light and living matter. For Bacteria this interaction serves three recognisable physiological functions: provision of energy, protection against excess radiation and signalling (for motility and gene expression). The chemical structure of the primary light-absorbing components in biology (the chromophores of photoactive proteins) is surprisingly simple: tetrapyrroles, polyenes and derivatised aromats are the most abundant… 

Engineering retinal-based phototrophy via a complementary photosystem in Synechocystis sp. PCC6803

This thesis focuses on expressing two retinal-based pumps, proteorhodopsin (PR) and Gloeobacter rhodopin (GR), into Synechocystis sp.

retinal-based phototrophy via a complementary photosystem in Synechocystis sp . PCC 6803

This work explains and discusses the concept of ‘direct conversion’ trough natural photosynthesis and discusses its limitations and potential further improvement.

A high resolution microscopy study of biological components for the incorporation in opto-electronic hybrid devices

Optical microscopy and scanning probe microscopy techniques have been utilised to acquire high resolution topography and fluorescence images of several biological samples. Applying these techniques

Biological HRPs in wastewater

Synechocystis: Not Just a Plug-Bug for CO2, but a Green E. coli

It is argued that this approach will be more likely to be successful if organisms such as Synechocystis are not perceived as mere chassis for CO2 fixation, but rather considered as the “green” E. coli.

UvA-DARE ( Digital Academic Repository ) Towards stable cyanobacterial cell factories

This dissertation aims to provide a history of web exceptionalism from 1989 to 2002, a period chosen in order to explore its roots as well as specific cases up to and including the year in which descriptions of “Web 2.0” began to circulate.

Corrigendum: Synechocystis: not just a plug-bug for CO2, but a green E. coli

This research presents a novel probabilistic procedure called “spot-spot analysis” that allows for real-time analysis of the response of the immune system to natural catastrophes.



The phycobilisome, a light-harvesting complex responsive to environmental conditions.

The phycobilisome, the light-harvesting apparatus of cyanobacteria and red algae, is discussed, which allows these organisms to efficiently utilize available light energy to drive photosynthetic electron transport and CO2 fixation.

Properties of a water-soluble, yellow protein isolated from a halophilic phototrophic bacterium that has photochemical activity analogous to sensory rhodopsin.

The absorption at 446 nm in the native protein appears to result from a 106-nm red shift of the chromophore induced by the protein, which is remarkably similar to those of sensory rhodopsin from Halobacterium.

A functional model for the role of cytochrome b559 in the protection against donor and acceptor side photoinhibition.

  • J. BarberJ. de Las Rivas
  • Chemistry, Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1993
This conclusion has been incorporated into a model that further suggests that in its high potential form the cytochrome primarily acts to protect against donor side photoinhibition due to increased lifetime of highly oxidized species as previously proposed by Thompson and Brudvig.

Photoinduced degradation of the D1 polypeptide in isolated reaction centers of photosystem II: evidence for an autoproteolytic process triggered by the oxidizing side of the photosystem.

  • C. ShiptonJ. Barber
  • Chemistry, Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1991
The results indicate that the photoinduced degradation of D1 (and D2) does not involve exogenous proteases but is most likely an autoproteolytic process and the photochemical damage giving rise to D1 and D2 degradation occurs on the oxidizing rather than the reducing side of photosystem II and involves photooxidation of the accessory pigments.

Transient proton uptake and release is associated with the photocycle of the photoactive yellow protein from the purple phototrophic bacterium Ectothiorhodospira halophila.

The results suggest that a basic residue which is normally buried in the protein interior is transiently exposed to solvent during the PYP photocycle and, as a consequence, undergoes a change in pK.

The eubacterium Ectothiorhodospira halophila is negatively phototactic, with a wavelength dependence that fits the absorption spectrum of the photoactive yellow protein

To the authors' knowledge, this is the first report of a true repellent response to light in a free-swimming eubacterium, since the blue light response in Escherichia coli and Salmonella typhimurium is unlikely to be a sensory process.

Electron-transfer reactions in manganese-depleted photosystem II.

The first global fit for electron donation in Mn-depleted PSII that accounts for the data over the pH range from 5 to 7.5 is obtained and the kinetics and yields of electron donation from cytochrome b559 and YD to P680+ will depend on the S2- or S3-state lifetime.