Photobacterium damselae ssp. piscicida: detection by direct amplification of 16S rRNA gene sequences and genotypic variation as determined by amplified fragment length polymorphism (AFLP).

@article{Kvitt2002PhotobacteriumDS,
  title={Photobacterium damselae ssp. piscicida: detection by direct amplification of 16S rRNA gene sequences and genotypic variation as determined by amplified fragment length polymorphism (AFLP).},
  author={Hagit Kvitt and Michal Ucko and A. Colorni and Costas Batargias and Amir Zlotkin and Wayne R. Knibb},
  journal={Diseases of aquatic organisms},
  year={2002},
  volume={48 3},
  pages={187-95}
}
A PCR protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16S rRNA gene sequences was developed. The procedure combines low annealing temperature that detects low titers of Photobacterium damselae but also related species, and high annealing temperature for the specific identification of P. damselae directly from infected fish. The PCR protocol was validated on 19 piscine isolates of P. damselae ssp. piscicida from different geographic regions (Japan, Italy, Spain, Greece and… CONTINUE READING
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