Photoaffinity labelling of the pea chloroplast transcriptional complex by nascent RNA in vitro.

@article{Khanna1991PhotoaffinityLO,
  title={Photoaffinity labelling of the pea chloroplast transcriptional complex by nascent RNA in vitro.},
  author={Navin C. Khanna and Sujata Lakhani and Krishna K. Tewari},
  journal={Nucleic acids research},
  year={1991},
  volume={19 18},
  pages={4849-55}
}
We have used photoaffinity labelling to examine the chloroplast RNA polymerase components which come into contact with nascent transcripts during the in vitro transcription of plastid DNA. The transcripts were synthesized in the presence of a photoactive analogue (4-thio UTP) and alpha-32P-ATP, using enriched pea chloroplast RNA polymerase preparation and a recombinant plasmid containing the plastid 16S rRNA promoter. Brief irradiation of the transcriptional complex crosslinked the photoactive… CONTINUE READING