Photoaffinity labeling of atrial natriuretic factor analog atriopeptin III with [gamma-32P]8N3GTP.

Abstract

We have determined that atrial natriuretic factor (ANF) analog atriopeptin III (AP III) is a guanosine 5'-triphosphate (GTP) binding protein as shown by photoaffinity labeling with the GTP-analog [gamma-32P]8N3GTP (azido-GTP). The photo-insertion was saturable at 40 microM azido-GTP. Half-maximal protection from photo-insertion was observed using 40-microM… (More)

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