Photoaffinity labeling of a protein kinase from bovine brain with 8-azidoadenosine 3',5'-monophosphate.

@article{Pomerantz1975PhotoaffinityLO,
  title={Photoaffinity labeling of a protein kinase from bovine brain with 8-azidoadenosine 3',5'-monophosphate.},
  author={A. Pomerantz and S. A. Rudolph and B. Haley and P. Greengard},
  journal={Biochemistry},
  year={1975},
  volume={14 17},
  pages={
          3858-62
        }
}
8-Azidoadenosine 3',5'-monophosphate (8-N3-cAMP) containing 32P has been used as a photoaffinity label specific for the adenosine 3',5'-monophosphate (cAMP) binding site(s) present in a partially purified preparation of soluble protein kinase from bovine brain. 8-N3-cAMP and cAMP were found to compete for the same binding site(s) in this preparation, as determined by a standard filter assay. When this protein preparation was equilibrated with [32P]-8-N3-cAMP, and then irradiated at 253.7 nm… Expand
Adenosine 3',5'-monophosphate receptor proteins in HeLa cells.
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