Photoaffinity Labeling of Mature and Precursor Forms of the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase after Expression in Escherichia coli.

Abstract

The small subunit (SSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) possesses a binding site that can be photoaffinity labeled with [(32)P]8-azidoadenosine 5' triphosphate (N(3)ATP). In the present study, photoaffinity labeling was used to compare the nucleotide analog binding properties of SSU in the Rubisco holoenzyme complex (holoE SSU… (More)

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@article{Klein1992PhotoaffinityLO, title={Photoaffinity Labeling of Mature and Precursor Forms of the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase after Expression in Escherichia coli.}, author={Robert R. Klein and Michael E. Salvucci}, journal={Plant physiology}, year={1992}, volume={98 2}, pages={546-53} }