Photoactivation of the CreER T2 recombinase for conditional site-specific recombination with high spatiotemporal resolution.

Abstract

We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells… (More)
DOI: 10.1089/zeb.2009.0632

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