Photoactivated Green Fluorescence Emission by Femtosecond Oscillator from Indole Solutions


We reported a novel femtosecond-laser-activated fluorescence emission from indole solutions upon excitation by the second harmonic wavelength of a femtosecond oscillator. A new absorption band around 400 nm and corresponding fluorescent band in the green domain were produced after the irradiation of femtosecond laser. This femtosecond-laser-activated luminescence process that allows the use of visible wavelength as a substitute for UV light to excite fluorescence from indole would extend applications based on indole chromophore. Furthermore, the photoactived emission can act as a fluorescence lifetime probe to measure the polarity in complex biological systems since it is polarity-sensitive. High performance liquid chromatography with fluorescence detector (HPLC-FLU) and high performance liquid chromatography with mass spectrometer (HPLC-MS) analysis demonstrate that the origin of the photoactivated fluorescence is new molecular species that generated in indole solution upon femtosecond laser irradiation.

DOI: 10.1007/s10895-011-0921-4

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@article{Wang2011PhotoactivatedGF, title={Photoactivated Green Fluorescence Emission by Femtosecond Oscillator from Indole Solutions}, author={Chen Wang and Lei Feng and Li Liu and Yuanlong Wang and Lingling Qiao and Jielei Ni and Jian Xu}, journal={Journal of Fluorescence}, year={2011}, volume={21}, pages={2185-2191} }