Phosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domain.

@article{Gustafson1995PhosphotyrosinedependentIO,
  title={Phosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domain.},
  author={Thomas A. Gustafson and Weiwei He and A Craparo and Cornelius Schaub and Thomas J. O'Neill},
  journal={Molecular and cellular biology},
  year={1995},
  volume={15 5},
  pages={2500-8}
}
The SHC proteins have been implicated in insulin receptor (IR) signaling. In this study, we used the sensitive two-hybrid assay of protein-protein interaction to demonstrate that SHC interacts directly with the IR. The interaction is mediated by SHC amino acids 1 to 238 and is therefore independent of the Src homology 2 domain. The interaction is dependent upon IR autophosphorylation, since the interaction is eliminated by mutation of the IR ATP-binding site. In addition, mutational analysis of… CONTINUE READING
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