Phosphorylation of the beta2-adrenergic receptor in plasma membranes by intrinsic GRK5.

@article{Tran2007PhosphorylationOT,
  title={Phosphorylation of the beta2-adrenergic receptor in plasma membranes by intrinsic GRK5.},
  author={Tuan M. Tran and Rasmus L Jorgensen and Richard B. Clark},
  journal={Biochemistry},
  year={2007},
  volume={46 50},
  pages={14438-49}
}
Characterization of the GRKs participating in the phosphorylation of the beta2-adrenergic receptor (beta2AR) have in part been limited by the lack of a simple cell-free assay with membrane-bound beta2AR and GRKs. We describe here a cell-free assay for GRK phosphorylation of the beta2AR in a postnuclear 600g fraction and washed membranes by intrinsic GRK activity using the GRK phosphosite-specific antibody that recognizes pS(355,356). Treatment of these cell-free preparations with 1.0 microM… CONTINUE READING
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