Phosphorylation of nitrogen regulator I of Escherichia coli induces strong cooperative binding to DNA essential for activation of transcription.

@article{Weiss1992PhosphorylationON,
  title={Phosphorylation of nitrogen regulator I of Escherichia coli induces strong cooperative binding to DNA essential for activation of transcription.},
  author={Valeria Weiss and F{\'e}lix Claverie-Mart{\'i}n and Boris Magasanik},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 11},
  pages={5088-92}
}
We studied the effect of phosphorylation of nitrogen regulator I (NRI) on its binding properties. Both phosphorylated and unphosphorylated NRI bind linearly to a single binding site but cooperatively to two adjacent binding sites. Cooperative binding of NRI is severely affected by phosphorylation: half-maximal binding of NRI-phosphate is at 20-fold lower concentrations than that of unphosphorylated NRI. This is more due to a huge increase in the cooperativity constant--which is the strength of… CONTINUE READING

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