Phosphorylation of mammalian translation initiation factor 5 (eIF5) in vitro and in vivo.

Abstract

Eukaryotic translation initiation factor 5 (eIF5) interacts with the 40S initiation complex (40S*eIF3*AUG*Met-tRNA(f)*eIF2*GTP) and, acting as a GTPase activating protein, promotes the hydrolysis of bound GTP. We isolated a protein kinase from rabbit reticulocyte lysates on the basis of its ability to phosphorylate purified bacterially expressed recombinant… (More)

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@article{Majumdar2002PhosphorylationOM, title={Phosphorylation of mammalian translation initiation factor 5 (eIF5) in vitro and in vivo.}, author={Romit Majumdar and Amitabha Bandyopadhyay and H. Deng and Umadas Maitra}, journal={Nucleic acids research}, year={2002}, volume={30 5}, pages={1154-62} }