Phosphorylation of lens-fiber connexins in lens organ cultures.

  title={Phosphorylation of lens-fiber connexins in lens organ cultures.},
  author={Jean X Jiang and Daniel A. Goodenough},
  journal={European journal of biochemistry},
  volume={255 1},
Lens gap junction proteins, connexins [1], are known to be phosphorylated in vivo. Phosphorylated states of connexins were examined in lens cultures to define in vitro models for the study of the functions of lens connexin phosphorylation in lens biology. In organ and differentiated cell primary cultures, chick lens-fiber connexins, connexin45.6 and connexin56, were labeled with [32P]orthophosphate. Pulse-chase experiments of lens organ cultures with [35S]methionine demonstrated that connexin45… CONTINUE READING