Phosphorylation of human Fen1 by cyclin-dependent kinase modulates its role in replication fork regulation

@article{Henneke2003PhosphorylationOH,
  title={Phosphorylation of human Fen1 by cyclin-dependent kinase modulates its role in replication fork regulation},
  author={Ghislaine Henneke and St{\'e}phane Koundrioukoff and Ulrich H{\"u}bscher},
  journal={Oncogene},
  year={2003},
  volume={22},
  pages={4301-4313}
}
Cyclin-dependent kinase (Cdk) Cdk1–Cyclin A can phosphorylate Flap endonuclease 1 (Fen1), a key-enzyme of the DNA replication machinery, in late S phase. Cdk1–cyclin A forms a complex in vitro and in vivo with Fen1. Furthermore, Fen1 phosphorylation is detected in vivo and depends upon Cdks activity. As a functional consequence of phosphorylation by Cdk1–Cyclin A in vitro, endo- and exonuclease activities of Fen1 are reduced whereas its DNA binding is not affected. Moreover, phosphorylation of… CONTINUE READING