Phosphorylation of farnesol by a cell-free system from Botryococcus braunii.

  title={Phosphorylation of farnesol by a cell-free system from Botryococcus braunii.},
  author={Hitoshi Inoue and Tatsumi Korenaga and Hiroshi Sagami and Takako Koyama and Kyozo Ogura},
  journal={Biochemical and biophysical research communications},
  volume={200 2},
Farnesol was incorporated into squalene as well as botryococcenes when the alcohol was fed to the culture of Botryococcus braunii B race strain. In in vitro experiments with a 10,000 x g supernatant of cell homogenate, squalene was synthesized from farnesyl diphosphate in the presence of NADPH or NADH, but botryococcenes were not synthesized under the same conditions. A 100,000 x g pelet fraction was able to phosphorylate farnesol to give its mono- and diphosphate esters in a CTP dependent… 
Metabolism of farnesol: phosphorylation of farnesol by rat liver microsomal and peroxisomal fractions.
It is demonstrated for the first time that rat liver microsomal and peroxisomal fractions are able to phosphorylate free farnesol to its diphosphate ester in a CTP dependent manner and can be oxidized to a prenyl aldehyde by an alcohol dehydrogenase (ADH).
Botryococcus braunii: a rich source for hydrocarbons and related ether lipids
The algal biodiversity, the chemical structures and biosynthesis of hydrocarbons and ether lipids and the biotechnological studies related to hydrocarbon production are reviewed.
Squalene – biochemistry, molecular biology, process biotechnology, and applications
The recent knowledge of squalene biochemistry, its molecular properties, and its physiological effects are summarized and applications in nutrition, pharmacy, and medicine are discussed.
Phosphorylation of farnesol in rat liver microsomes: properties of farnesol kinase and farnesyl phosphate kinase.
It is demonstrated that rat liver microsomes contain two enzymes for the consecutive phosphorylation of farnesol to farnesyl-PP, and this reaction probably takes place on the outer, cytoplasmic surface of microsomal vesicles.
Botryococcus braunii: A Renewable Source of Hydrocarbons and Other Chemicals
This review details the various facets of biotechnology of B. braunii, including its microbiology and physiology; production of hydrocarbons and other compounds by the alga; methods of culture; downstream recovery and processing of algal hydrocarols; and cloning of the algal genes into other microorganisms.
Farnesol is utilized for isoprenoid biosynthesis in plant cells via farnesyl pyrophosphate formed by successive monophosphorylation reactions.
  • L. Thai, J. Rush, C. J. Waechter
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1999
These results document the presence of at least two CTP-mediated kinases that provide a mechanism for the utilization of F-OH and GG-OH for the biosynthesis of isoprenoid lipids and protein isopranylation.