Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae

@inproceedings{Boeckmann2013PhosphorylationOC,
  title={Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae},
  author={Lars Boeckmann and Yoshimitsu Takahashi and Wei-Chun Au and Prashant K. Mishra and John S. Choy and Anthony R Dawson and May Y. Szeto and Timothy J. Waybright and Christopher D Heger and Christopher McAndrew and Paul K. Goldsmith and Timothy D. Veenstra and Richard E. Baker and Munira A. Basrai},
  booktitle={Molecular biology of the cell},
  year={2013}
}
The centromeric histone H3 variant (CenH3) is essential for chromosome segregation in eukaryotes. We identify posttranslational modifications of Saccharomyces cerevisiae CenH3, Cse4. Functional characterization of cse4 phosphorylation mutants shows growth and chromosome segregation defects when combined with kinetochore mutants okp1 and ame1. Using a phosphoserine-specific antibody, we show that the association of phosphorylated Cse4 with centromeres increases in response to defective… CONTINUE READING