Phosphorylation of a neuronal-specific beta-tubulin isotype.

@article{DazNido1990PhosphorylationOA,
  title={Phosphorylation of a neuronal-specific beta-tubulin isotype.},
  author={Javier D{\'i}az-Nido and Luis J Serrano and Carlos L{\'o}pez-Ot{\'i}n and Jo{\"e}l Vandekerckhove and Jes{\'u}s {\'A}vila},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 23},
  pages={13949-54}
}
Adult rats were intracraneally injected with [32P] phosphate and brain microtubules isolated. The electrophoretically purified, in vivo phospholabeled, beta-tubulin was digested with the V8-protease and the labeled peptide purified by reversed-phase liquid chromatography. Its amino acid sequence corresponds to the COOH-terminal sequence of a minor neuronal beta 3-tubulin isoform from chicken and human. The phosphorylation site was at serine 444. A synthetic peptide with sequence… CONTINUE READING

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