Phosphorylation of Sindbis virus nsP3 in vivo and in vitro.
@article{Li1990PhosphorylationOS, title={Phosphorylation of Sindbis virus nsP3 in vivo and in vitro.}, author={G. Li and M. W. La Starza and W. R. Hardy and J. Strauss and C. Rice}, journal={Virology}, year={1990}, volume={179 1}, pages={ 416-27 } }
nsP3 is one of four viral nonstructural proteins required for RNA replication of Sindbis virus. In this report, post-translational modifications of nsP3 which occur in both vertebrate and mosquito cell cultures have been examined. In pulse-chase experiments, analyzed by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, nsP3 was initially observed as a single species (termed nsP3a, approximately 76 kDa) which was gradually converted to slower mobility forms… CONTINUE READING
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References
SHOWING 1-10 OF 69 REFERENCES
Processing the nonstructural polyproteins of sindbis virus: nonstructural proteinase is in the C-terminal half of nsP2 and functions both in cis and in trans.
- Biology, Medicine
- Journal of virology
- 1989
- 203
- PDF
Evidence that Sindbis virus NSP2 is an autoprotease which processes the virus nonstructural polyprotein.
- Biology, Medicine
- Virology
- 1989
- 106
Processing the nonstructural polyproteins of Sindbis virus: study of the kinetics in vivo by using monospecific antibodies.
- Biology, Medicine
- Journal of virology
- 1988
- 68
- PDF
Cleavage‐site preferences of Sindbis virus polyproteins containing the non‐structural proteinase. Evidence for temporal regulation of polyprotein processing in vivo.
- Biology, Medicine
- The EMBO journal
- 1990
- 145
Semliki Forest virus-specific non-structural protein nsP3 is a phosphoprotein.
- Biology, Medicine
- The Journal of general virology
- 1988
- 111
Synthesis and processing of the nonstructural polyproteins of several temperature-sensitive mutants of Sindbis virus.
- Biology, Medicine
- Virology
- 1990
- 43
Site-specific phosphorylation regulates the transcriptive activity of vesicular stomatitis virus NS protein.
- Biology, Medicine
- Journal of virology
- 1982
- 56
- PDF
The synthesis of Sendai virus polypeptides in infected cells. III. Phosphorylation of polypeptides.
- Biology, Medicine
- Virology
- 1977
- 110
Sindbis virus proteins nsP1 and nsP2 contain homology to nonstructural proteins from several RNA plant viruses.
- Biology, Medicine
- Journal of virology
- 1985
- 175
- PDF