Phosphorylation of Sindbis virus nsP3 in vivo and in vitro.

  title={Phosphorylation of Sindbis virus nsP3 in vivo and in vitro.},
  author={G. Li and M. W. La Starza and W. R. Hardy and J. Strauss and C. Rice},
  volume={179 1},
  • G. Li, M. W. La Starza, +2 authors C. Rice
  • Published 1990
  • Biology, Medicine
  • Virology
  • nsP3 is one of four viral nonstructural proteins required for RNA replication of Sindbis virus. In this report, post-translational modifications of nsP3 which occur in both vertebrate and mosquito cell cultures have been examined. In pulse-chase experiments, analyzed by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, nsP3 was initially observed as a single species (termed nsP3a, approximately 76 kDa) which was gradually converted to slower mobility forms… CONTINUE READING
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