Phosphorylation of Neuroglycan C, a Brain-specific Transmembrane Chondroitin Sulfate Proteoglycan, and Its Localization in the Lipid Rafts*

@article{Yamauchi2002PhosphorylationON,
  title={Phosphorylation of Neuroglycan C, a Brain-specific Transmembrane Chondroitin Sulfate Proteoglycan, and Its Localization in the Lipid Rafts*},
  author={Shinobu Yamauchi and Yoshihito Tokita and Sachiko Aono and Fumiko Matsui and Takuya Shuo and Hidenori Ito and Kanefusa Kato and Kohji Kasahara and Atsuhiko Oohira},
  journal={The Journal of Biological Chemistry},
  year={2002},
  volume={277},
  pages={20583 - 20590}
}
Neuroglycan C (NGC) is a brain-specific transmembrane chondroitin sulfate proteoglycan. In the present study, we examined whether NGC could be phosphorylated in neural cells. On metabolic labeling of cultured cerebral cortical cells from the rat fetus with 32Pi, serine residues in NGC were radiolabeled. Some NGC became detectable in the raft fraction from the rat cerebrum, a signaling microdomain of the plasma membrane, with cerebral development. NGC from the non-raft fraction, not the raft… 

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Glycosylation Site for Chondroitin Sulfate on the Neural Part-time Proteoglycan, Neuroglycan C*
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Expression and identification of a new splice variant of neuroglycan C, a transmembrane chondroitin sulfate proteoglycan, in the human brain
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The expression of NGC in the human brain, mainly in the hippocampus, was studied and some observations were confirmed by conducting experiments using rat brain, and NGC‐IV, which was first found in the present study, had the shortest cytoplasmic domain among the four variants.
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It is reported that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture, and both protein kinase C (PKC) inhibitors and phosphatidylinositol 3-kinase (PI3K) inhibitors attenuated the NGC-mediated neurites outgrowth in a dose-dependent manner, suggesting that NGC promotes neuriteOutgrowth via PI3K and PKC pathways.
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One‐thousand‐and‐one substrates of protein kinase CK2?
  • F. Meggio, L. Pinna
  • Biology
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2003
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References

SHOWING 1-10 OF 65 REFERENCES
Genomic Organization and Expression Pattern of Mouse Neuroglycan C in the Cerebellar Development*
TLDR
Western blot analysis demonstrated that, although N GC in the immature cerebellum existed in a proteoglycan form, most NGC in the mature Cerebellum did not bear chondroitin sulfate chain(s), indicating that NGC is a typical part-time proteoglyca.
Involvement of Gangliosides in Glycosylphosphatidylinositol-anchored Neuronal Cell Adhesion Molecule TAG-1 Signaling in Lipid Rafts*
TLDR
Observations suggest that glycosphingolipids are involved in TAG-1-mediated signaling in lipid rafts of the cerebellum.
A membrane‐bound heparan sulfate proteoglycan that is transiently expressed on growing axons in the rat brain
TLDR
The data suggest that H5‐PG is a glycoconjugate on axonal surface that is involved in axonal outgrowth and/or synaptogenesis in the central nervous system.
A substrate of ecto-protein kinase is microtubule-associated protein 1B in cortical cell cultures undergoing synaptogenesis.
TLDR
The possibility that the phosphorylation of extracellular domains of microtubule-associated protein (MAP1B) is involved in synaptogenesis between cortical neurons is suggested.
Molecular interactions of neural chondroitin sulfate proteoglycans in the brain development.
TLDR
This review mainly summarizes recent studies on the involvement of these three classes of proteoglycan in cell-cell and cell-substratum interactions during the brain development.
The glypican family of heparan sulfate proteoglycans: major cell-surface proteoglycans of the developing nervous system.
TLDR
In Drosophila, the effects of mutations in the daily gene suggest a role for members of the glypican family in regulating cell cycle progression during the transition of neural cells from proliferation to neuronal differentiation.
Surface Phosphorylation by Ecto‐Protein Kinase C in Brain Neurons: A Target for Alzheimer's β‐Amyloid Peptides
TLDR
The discovery that ecto‐PKC‐mediated protein phosphorylation serves as a target for β‐amyloid peptides at the very site they operate, i.e., at the neuronal cell surface, opens a new research direction in the investigation of molecular events that play a role in the etiology of developmental disabilities and neurodegenerative disorders.
Association of Src Family Tyrosine Kinase Lyn with Ganglioside GD3 in Rat Brain
TLDR
Observations suggest that GD3 may regulate Lyn in a caveolae-like domain on brain cell membranes onbrain cell membranes, as suggested by sucrose density gradient analysis.
...
...