Phosphorylation of Mg(2+)-dependent protein phosphatase alpha (type 2C alpha) by casein kinase II.

@article{Kobayashi1993PhosphorylationOM,
  title={Phosphorylation of Mg(2+)-dependent protein phosphatase alpha (type 2C alpha) by casein kinase II.},
  author={Tomoo Kobayashi and Shin-ichiro Kanno and Takeshi Terasawa and Tadashi Murakami and Motoko Ohnishi and Kenzo Ohtsuki and Akira Hiraga and Shinri Tamura},
  journal={Biochemical and biophysical research communications},
  year={1993},
  volume={195 1},
  pages={484-9}
}
In this study we show that rat Mg(2+)-dependent protein phosphatase alpha (MPP alpha) expressed in Saccharomyces cerevisiae cells was phosphorylated on serine residues in vivo. The recombinant rat MPP alpha purified from Escherichia coli cells harboring an expression vector was phosphorylated in vitro by casein kinase II, but not by casein kinase I, to 1.5 mol phosphate per mol enzyme protein. Analysis by phosphopeptide mapping and amino acid analysis suggested that the sites of both in vivo… CONTINUE READING