Phosphorylation of E2F-1 by cyclin A-cdk2.

  title={Phosphorylation of E2F-1 by cyclin A-cdk2.},
  author={Mamiko Kitagawa and Hideaki Higashi and Ikuko Suzuki-Takahashi and Katsumori Segawa and Steven K Hanks and Yoichi Taya and Shintaro Nishimura and Akihiko Okuyama},
  volume={10 2},
Transcription factor E2F-1 has a putative consensus sequence for phosphorylation by cyclin dependent kinase (Ser-Pro-X-Lys/Arg). Therefore, we studied the phosphorylation of E2F-1 in vivo and in vitro and its biological functions. E2F-1 was prepared by immunoprecipitation with anti-E2F-1 antibody from IMR32 lysates and was effectively phosphorylated by human cyclin A-cdk2 which was expressed in insect cells using baculovirus system. GST-E2F-1 was phosphorylated by cyclin A-cdk2 more efficiently… CONTINUE READING


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