Phosphorothioate hammerhead ribozymes targeting a conserved sequence in the V3 loop region inhibit HIV-1 entry.

@article{Zhang1998PhosphorothioateHR,
  title={Phosphorothioate hammerhead ribozymes targeting a conserved sequence in the V3 loop region inhibit HIV-1 entry.},
  author={Xiaohua Zhang and Yoshiori Iwatani and Toshio Shimayama and Ryo Yamada and Atsushi Koito and Yong Xu and Hiroyuki Sakai and Takamichi Uchiyama and T Hattori},
  journal={Antisense & nucleic acid drug development},
  year={1998},
  volume={8 6},
  pages={
          441-50
        }
}
Seven different phosphorothioate DNA-RNA chimeric hammerhead ribozymes (RzV3-nT, n = 1-7) targeted against the V3 loop region of HIV-1 were synthesized. Two of these, RzV3-1T and RzV3-3T, efficiently cleaved transcribed envelope RNA of HXB2 in vitro. The target sequence of RzV3-1T belongs to a conserved region and is completely identical in the HIV-1 HXB2, NL432, and ADA strains. Furthermore, RzV3-1T cleaved the envelope RNA of HIV-1 SF162 with a single base substitution in the distal site. U87… CONTINUE READING