Phosphoric acid as a matrix additive for MALDI MS analysis of phosphopeptides and phosphoproteins.

@article{Kjellstrm2004PhosphoricAA,
  title={Phosphoric acid as a matrix additive for MALDI MS analysis of phosphopeptides and phosphoproteins.},
  author={Sven Kjellstr{\"o}m and Ole N\orregaard Jensen},
  journal={Analytical chemistry},
  year={2004},
  volume={76 17},
  pages={5109-17}
}
Phosphopeptides are often detected with low efficiency by MALDI MS analysis of peptide mixtures. In an effort to improve the phosphopeptide ion response in MALDI MS, we investigated the effects of adding low concentrations of organic and inorganic acids during peptide sample preparation in 2,5-dihydroxybenzoic acid (2,5-DHB) matrix. Phosphoric acid in combination with 2,5-DHB matrix significantly enhanced phosphopeptide ion signals in MALDI mass spectra of crude peptide mixtures derived from… CONTINUE READING