Phosphoproteomic profiling reveals vasopressin-regulated phosphorylation sites in collecting duct.

@article{Bansal2010PhosphoproteomicPR,
  title={Phosphoproteomic profiling reveals vasopressin-regulated phosphorylation sites in collecting duct.},
  author={Amar D Bansal and Jason D. Hoffert and Trairak Pisitkun and Shelly Hwang and C L Chou and Emily S. Boja and Guanghui Wang and Mark A. Knepper},
  journal={Journal of the American Society of Nephrology : JASN},
  year={2010},
  volume={21 2},
  pages={303-15}
}
Protein phosphorylation is an important component of vasopressin signaling in the renal collecting duct, but the database of known phosphoproteins is incomplete. We used tandem mass spectrometry to identify vasopressin-regulated phosphorylation events in isolated rat inner medullary collecting duct (IMCD) suspensions. Using multiple search algorithms to identify the phosphopeptides from spectral data, we expanded the size of the existing collecting duct phosphoproteome database from 367 to 1187… CONTINUE READING