Phosphoproteomic analysis of peptides.

  title={Phosphoproteomic analysis of peptides.},
  author={Mark A. Baker},
  journal={Methods in molecular biology},
Posttranslational modifications represent a major mechanism by which cells control molecular events. Sample preparation is one of the biggest keys in terms of proteomic analysis, and especially when looking at phosphoproteomics. Here we describe two techniques that are used to identify phosphopeptides. These are Titanium Dioxide and Immobilized Metal Affinity Chromatography (IMAC) which enrich for phosphopeptides. These enrichment techniques typically often identify ~35 % of the same and ~65… CONTINUE READING