Phosphoproteome analysis of formalin-fixed and paraffin-embedded tissue sections mounted on microscope slides.

@article{Wakabayashi2014PhosphoproteomeAO,
  title={Phosphoproteome analysis of formalin-fixed and paraffin-embedded tissue sections mounted on microscope slides.},
  author={Masaki Wakabayashi and Hiroki Yoshihara and Takeshi Masuda and Mai Tsukahara and Naoyuki Sugiyama and Yasushi Ishihama},
  journal={Journal of proteome research},
  year={2014},
  volume={13 2},
  pages={915-24}
}
Formalin-fixed and paraffin-embedded (FFPE) sections mounted on microscope slides are one of the largest available resources for retrospective research on various diseases, but quantitative phosphoproteome analysis of FFPE sections has never been achieved because of the extreme difficulty of procuring sufficient phosphopeptides from the limited amounts of proteins on the slides. Here, we present the first protocol for quantitative phosphoproteome analysis of FFPE sections by utilizing phase… CONTINUE READING
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