Phosphoprotein inhibition of calcium-stimulated exocytosis in sea urchin eggs

@article{Whalley1991PhosphoproteinIO,
  title={Phosphoprotein inhibition of calcium-stimulated exocytosis in sea urchin eggs},
  author={T D Whalley and I R Crossley and M Whitaker},
  journal={The Journal of Cell Biology},
  year={1991},
  volume={113},
  pages={769 - 778}
}
We have investigated the role of protein phosphorylation in the control of exocytosis in sea urchin eggs by treating eggs with a thio-analogue of ATP. ATP gamma S (adenosine 5'-O-3-thiotriphosphate) is a compound which can be used as a phosphoryl donor by protein kinases, leading to irreversible protein thiophosphorylation (Gratecos, D., and E.H. Fischer. 1974. Biochem. Biophys. Res. Commun. 58:960-967). Microinjection of ATP gamma S inhibits cortical granule exocytosis, but has no effect on… CONTINUE READING