Phosphopeptide mapping and identification of phosphorylation sites.

This unit describes a procedure for proteolytic digestion of a (32)P-labeled protein, followed by separation of the digestion products in two dimensions on a TLC plate, which gives rise to a phosphopeptide map. Phosphopeptides present on the TLC plate are visualized by autoradiography. These maps give information about the number of phosphate-containing… CONTINUE READING