Phosphonylmethoxyalkylpurines and -pyrimidines as inhibitors of African swine fever virus replication in vitro.

  title={Phosphonylmethoxyalkylpurines and -pyrimidines as inhibitors of African swine fever virus replication in vitro.},
  author={Carmen Gil-Fern{\'a}ndez and Dolores Garc{\'i}a-Villal{\'o}n and Erik De Clercq and Ivan Rosenberg and Antonín Holý},
  journal={Antiviral research},
  volume={8 5-6},

Efficacy of phosphonylmethoxyalkyl derivatives of adenine in experimental herpes simplex virus and vaccinia virus infections in vivo

Most dramatic were the effects shown by the compounds in mice inoculated intracerebrally with HSV-1,HSV-2, or TK- HSV -1, in which all three compounds given intraperitoneally at a dose of 50 or 100 mg/kg per day effected a significant reduction in the mortality rate of HSv-1-infected mice.

HPMPC (cidofovir), PMEA (adefovir) and Related Acyclic Nucleoside Phosphonate Analogues: A Review of their Pharmacology and Clinical Potential in the Treatment of Viral Infections

Preclinical data on the efficacy of PMPA in animal retrovirus models point to its potential usefulness against HIV infections, when given either prophylactically or therapeutically in the treatment of established HIV infections.

Acyclic adenine nucleoside phosphonates in plasma determined by high-performance liquid chromatography with fluorescence detection.

This method proved useful in accurately determining low PMEA concentrations in sera from PMEA-treated monkeys and cats and should be applicable to the quantification of PMEA, HPMPA, and FPMPA in plasma and urine of humans treated with any of these drugs.

Efficacy of 9-(2-phosphonylmethoxyethyl)adenine in the therapy of TK+ and TK- herpes simplex virus experimental keratitis.

Treatment with PMEA 0.2% eyedrops reduced the severity of keratitis caused by the TK- HSV-1 (P less than 0.05), whereas BVDU 0.3% and PMEA 1.2%.

Efficacy of (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine in various models of herpes simplex virus infection in mice

The most remarkable feature of HPMPC was that a single administration of the compound, even as late as 4 days after infection, conferred significant protection against HSV-1 orHSV-2 infection.

Inhibition of African swine fever virus in cultured swine monocytes by phosphonoacetic acid (PAA) and by phosphonoformic acid (PFA)

With prolonged exposure to PAA or PFA (beyond 1 week), ASFV could not be recovered from infected MO cultures, but the cytostatic and virus-suppressive effects of PAA and PFA could be reversed.



Novel acyclic adenosine analogs inhibit Epstein-Barr virus replication

Results indicate that (S)-HPMPA and PMEA are potent and selective anti-EBV agents in vitro.

Intracellular phosphorylation of broad-spectrum anti-DNA virus agent (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine and inhibition of viral DNA synthesis.

The selectivity of (S)-HPMPA as an antiviral agent cannot be attributed to a differential phosphorylation by virus-infected or uninfected cells but resides in a specific inhibitory effect on viral DNA synthesis.

Effect of disodium phosphonoacetate and iododeoxyuridine on the multiplication of African swine fever virus in vitro.

Inhibition of ASFV replication by PAA suggests that this virus, like other herpesviruses, involves a virus-specific DNA polymerase in its replication mechanism.

A novel selective broad-spectrum anti-DNA virus agent

It is reported that, in mice and rabbits in vivo, the compound is effective against both local and systemic infections with herpes simplex virus type 1, including herpetic keratitis caused by a TK− mutant which is resistant to the classical anti-herpes drugs.

New agents active against African swine fever virus

Megalomycin C was the most active of the four agents with respect to the concentration of compound that blocked the formation of infectious virus by 50%.

Synthesis of 9-(2-Phosphinomethoxyethyl)adenine and Related Compounds

Alkyl 2-chloroethoxymethyl(diethoxymethyl)phosphinates VII and XIII were prepared by reaction of silyl esters of dialkoxymethylphosphinic acid with 2-chloroethyl chloromethyl ether. Adenine was

Effect of chloroquine on African swine fever virus infection.

The results support the hypothesis that ASFV enters the cells by adsorptive endocytosis and not by fusion with the plasma membrane.

Inhibition of African swine fever (ASF virus replication by phosphonoacetic acid.

Phosphonoacetic acid (PAA) inhibits the multiplication of African swine fever (ASF) virus in VERO cells. The observed inhibition of the in vivo DNA synthesis could be related to the in vitro

Plaque Formation by African Swine Fever Virus

It is found that at least two isolates of African swine fever virus consistently produce plaques in monolayers of PK cells under solid overlays, which has led to the development of a quantitative technique using any of three PK cell strains developed by us during a period of 3 years.