Phosphonates to Phosphate : A Functional Annotation of the Essential Genes of the Phn Operon in Escherichia coli
@inproceedings{Kamat2013PhosphonatesTP, title={Phosphonates to Phosphate : A Functional Annotation of the Essential Genes of the Phn Operon in Escherichia coli}, author={Siddhesh S. Kamat and Howard J. Williams and Frank M. Raushel}, year={2013} }
The reaction mechanism for the enzymatic conversion of methyl phosphonate to phosphate and methane in Escherichia coli has eluded researchers over the last three decades despite significant genetic and in vivo studies. The phn operon governs the C-P lyase activity in E. coli. The essential genes within the phn operon are phnGHIJKLM. The proteins encoded by phnGHM were over-expressed in E. coli and purified to homogeneity using standard protocols. The proteins encoded by phnIJKL were soluble…
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