Phosphomannose isomerase: An efficient selectable marker for plant transformation

@article{Reed2001PhosphomannoseIA,
  title={Phosphomannose isomerase: An efficient selectable marker for plant transformation},
  author={Janet E Reed and Laubra Privalle and Marie Louise Powell and Mohamed Meghji and John Dawson and Erik M. Dunder and Janet Sutthe and Allan Wenck and Karen L Launis and Catherine Kramer and Yongjian Chang and Genevi{\`e}ve Hansen and Martha Wright},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2001},
  volume={37},
  pages={127-132}
}
Phosphomannose isomerase (PMI) catalyzes the reversible interconversion of mannose 6-phosphate and fructose 6-phosphate. Plant cells lacking this enzyme are incapable of surviving on synthetic medium containing mannose as a carbon source. Maize, wheat and barley plants, genetically modified to express the Escherichia coli manA gene (pmi) under the control of a plant promoter, were able to survive selection on mannose-containing medium. Transformation frequencies averaged 45% for maize… CONTINUE READING

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