Phospholipid composition of isolated guinea pig sperm outer acrosomal membrane and plasma membrane during capacitation in vitro.

@article{Stojanoff1988PhospholipidCO,
  title={Phospholipid composition of isolated guinea pig sperm outer acrosomal membrane and plasma membrane during capacitation in vitro.},
  author={A Stojanoff and Harold Bourne and Amy G. Andrews and Ross V. Hyne},
  journal={Gamete research},
  year={1988},
  volume={21 3},
  pages={297-311}
}
After capacitation of guinea pig spermatozoa in vitro, the plasma membrane was mechanically separated from the spermatozoa in the presence or absence of HgCl2 and subsequently isolated by density gradient centrifugation. Examination of the spermatozoa by electron microscopy after homogenization in the presence of HgCl2 revealed that plasma membrane was removed only from the acrosomal region and remained predominantly intact posterior to the equatorial segment of the sperm head, as well as the… CONTINUE READING