Phospholipid and cation activation of chimaeric choline/ethanolamine phosphotransferases.

  title={Phospholipid and cation activation of chimaeric choline/ethanolamine phosphotransferases.},
  author={Christopher R McMaster and Sherry C. Morash and R M Bell},
  journal={The Biochemical journal},
  volume={313 ( Pt 3)},
The Saccharomyces cerevisiae CPT1 and EPT1 genes encode for a cholinephosphotransferase (CPT) and choline/ethanolaminephosphotransferase, respectively. Both Cpt1p and Ept1p activities display an absolute requirement for cations and phospholipids. A mixed-micelle assay was employed to determine cation and lipid activators of parental and chimaeric Cpt1p/Ept1p enzymes to gain insight into their mechanism(s) of activation. Mg2+, Mn2+ and Co2+ were the only cations capable of activating Cpt1p and… CONTINUE READING