Phospholipid—Calcium Phosphate Complex: Enhanced Calcium Migration in the Presence of Phosphate

  title={Phospholipid—Calcium Phosphate Complex: Enhanced Calcium Migration in the Presence of Phosphate},
  author={J M Cotmore and George Jr. Nichols and Roy E. Wuthier},
  pages={1339 - 1341}
In the presence of acidic phospholipids, inorganic phosphate greatly enhances the net migration of calcium ions from the aqueous phase to the organic phase, an effect that does not occur at less than the physiological pH. The calcium complex in the organic phase is shown by electron microscopy to consist of spherules, composed of stoichiometric amount of calcium, inorganic phosphate, and phospholipid. The demonstration of complex formation between calcium phosphate and acidic phospholipids adds… 

Phosphate modulation of calcium transport by a calcium-phospholipid-phosphate complex of calcifying tissues

It was concluded that the Ca-PS-Pi complex acted as an ionophore and that the presence of coordinated Ca and P atoms facilitated Pi modulation of Ca2+ transport.

Extraction of a calcium-phospholipid-phosphate complex from bone

Evidence is presented to show that the complex was isolated by a modified Folch extraction employing ultrasonic disruption of cellular material and is a natural constituent of bone rather than one created artifcatually during extraction.

An ultrastructural study of the effects of acidic phospholipid substitutions on calcium phosphate precipitation in anionic liposomes

The data suggest that PS may be localized preferentially on the outer membrane surface in the presence of DCP but concentrated on the inner aspect in its absence.

The role of synthetic and bone extracted Ca-phospholipid-PO4 complexes in hydroxyapatite formation

The evidence supports the possiblity that a similar complex is responsible for the initial mineralization in matrix vesicles, and suggests that the complex isolated from bone was formed in a manner similar to the complexes in the authors' in vitro experiments.

Structural studies of a phosphatidyl serine-amorphous calcium phosphate complex

The results suggest there is a movement in the nitrogen atom of the phosphatidyl serine which approaches the calcium atom in the mineral phase, which would link the membrane structure of the vesicle to theructure of the mineral in a way that could explain some of its roles in biomineralization.

Fusion of erythrocyte ghosts induced by calcium phosphate. Kinetic characteristics and the role of Ca2+, phosphate and calcium-phosphate complexes.

Results indicated that fusion-prone conditions were induced prior to calcium phosphate precipitation, and suggested that membrane aggregation, and hence fusion, is facilitated by the formation of crystalline calcium phosphate nucleating on the ghost membrane.

Effect of phospholipids on the transformation of amorphous calcium phosphate to hydroxyapatitein vitro

The conversion of amorphous calcium phosphate to crystalline hydroxyapatite (HA) was studied in vitro in the presence or absence of phosphatidyl serine (PS) and other phospholipids, adding further evidence that lipids may play a role in the control of normal mineralization in vivo.

Persistence of complexed acidic phospholipids in rapidly mineralizing tissues is due to affinity for mineral and resistance to hydrolytic attack:In Vitro data

Evidence is supplied that complexed acidic phospholipids can persist in mineralizing tissues both because of their affinity for HA andBecause of their resistance to hydrolysis by phospholIPase A2.

Mineralization of Annexin-5-containing Lipid-Calcium-Phosphate Complexes

Nucleators based on amorphous calcium phosphate were prepared with PS alone, PS + phosphatidylethanolamine (PE), or PS + PE and other MV lipids and Anx-5 proved to be critical for nucleation of mineral formation.

Analysis and Molecular Modeling of the Formation, Structure, and Activity of the Phosphatidylserine-Calcium-Phosphate Complex Associated with Biomineralization*

Findings clarify the process of PS-CPLX formation, reveal details of its structure, and provide insight into its role as a nucleator of crystalline calcium phosphate mineral formation.



The Binding of Calcium in Mixtures of Phospholipids.∗

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Summary The binding of calcium in phos-pholipid mixtures is very dependent on the pH, it being a quantitative reflection of the types of dissociable groups in the phospho-lipids present. As the pH


The study of phosphatidylserine shows that ultrasonicated dispersions of this acidic lipid contain micelles with an average molecular weight of 4 x 106, and all of the ionogenic polar groups are readily available for titration, and are therefore probably present on the micellar surface.

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It is suggested that such reactions with cell membrane phospholipids may underlie inhibitory effects of LA on cellular ion fluxes and provide a chemical basis for anesthetic action.

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It is suggested that matrix vesicles are derived from cells and that they may play a role in initiating calcification at the epiphysis.


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