Phospholipase D1 is located and activated by protein kinase C alpha in the plasma membrane in 3Y1 fibroblast cell.

@article{Kim1999PhospholipaseDI,
  title={Phospholipase D1 is located and activated by protein kinase C alpha in the plasma membrane in 3Y1 fibroblast cell.},
  author={Yonghwan Kim and J. E. Kim and Sookjin Lee and Taehoon G. Lee and Jihun Kim and Jong Bae Park and Jung Min Han and Su Kil Jang and Pann Ghill Suh and Sung Ho Ryu},
  journal={Biochimica et biophysica acta},
  year={1999},
  volume={1436 3},
  pages={319-30}
}
The subcellular location of phospholipase D1 (PLD1) and its activation by protein kinase C alpha (PKC alpha) were examined by subcellular fractionation and by microscopic observation of green fluorescent protein-fused PLD1 (GFP-PLD1) or PKC alpha (GFP-PKC alpha) in fibroblastic 3Y1 cells. Major PLD1 immunoreactivity and PKC alpha-stimulated PLD activity segregated with a plasma membrane marker, even though a significant amount was co-fractionated with markers for endoplasmic reticulum (ER) and… CONTINUE READING
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