Phosphoinositide profiling in complex lipid mixtures using electrospray ionization mass spectrometry

Abstract

Phosphoinositides (phosphorylated derivatives of phosphatidylinositol, PI) are versatile intracellular signaling lipids whose occurrence in low concentrations complicates direct mass measurements. Here we present a sensitive method to detect, identify and quantify phosphatidylinositol phosphate (PIP) and phosphatidylinositol bisphosphate (PIP2) with… (More)
DOI: 10.1038/nbt837

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