Phosphoinositide-containing polymerized liposomes: stable membrane-mimetic vesicles for protein-lipid binding analysis.

@article{Ferguson2005PhosphoinositidecontainingPL,
  title={Phosphoinositide-containing polymerized liposomes: stable membrane-mimetic vesicles for protein-lipid binding analysis.},
  author={Colin Ferguson and Robyn D James and Cleve S Bigman and Donnie A Shepard and Yasmina Noubia Abdiche and Phinikoula S. Katsamba and David G. Myszka and Glenn D. Prestwich},
  journal={Bioconjugate chemistry},
  year={2005},
  volume={16 6},
  pages={1475-83}
}
Stable phosphoinositide (PIP(n))-containing liposomes were prepared using polydiacetylene photochemistry. Tethered pentacosadiynyl inositol polyphosphate (InsP(n)) analogues of Ins(1,3,4)P(3), Ins(1,4,5)P(3), and Ins(1,3,4,5)P(4) were synthesized, incorporated into vesicles made up of diyne-phosphatidylcholine and -phosphatidylethanolamine, and polymerized by UV irradiation. The polymerized liposome nanoparticles showed markedly increased stability over conventional PIP(n)-containing vesicles… CONTINUE READING