Phosphoglucose isomerase mutant of Rhizobium meliloti.

  title={Phosphoglucose isomerase mutant of Rhizobium meliloti.},
  author={Alicia Arias and Carlos Cerve{\~n}ansky and A E Gardiol and Gloria Mart{\'i}nez-Drets},
  journal={Journal of bacteriology},
  volume={137 1},
A mutant strain of complex phenotype was selected in Rhizobium meliloti after nitrosoguanidine mutagenesis. It failed to grow on mannitol, sorbitol, fructose, mannose, ribose, arabitol, or xylose, but grew on glucose, maltose, gluconate, L-arabinose, and many other carbohydrates. Assay showed the enzyme lesion to be in phosphoglucose isomerase (pgi), and revertants, which were of normal growth phenotype, contained the enzyme again. Nonpermissive substrates such as fructose and xylose prevented… CONTINUE READING