Phosphoflow-Based Evaluation of Mek Inhibitors as Small-Molecule Therapeutics for B-Cell Precursor Acute Lymphoblastic Leukemia

@inproceedings{George2015PhosphoflowBasedEO,
  title={Phosphoflow-Based Evaluation of Mek Inhibitors as Small-Molecule Therapeutics for B-Cell Precursor Acute Lymphoblastic Leukemia},
  author={Aswathi Ann George and Helicia Paz and Fei Fei and Jonathan D Kirzner and Yong-mi Kim and Nora Heisterkamp and Hisham Abdel-Azim and Linda J Bendall},
  booktitle={PloS one},
  year={2015}
}
Upstream mutations that lead to constitutive activation of Erk in B-cell precursor acute lymphoblastic leukemia (BCP-ALL) are relatively common. In the era of personalized medicine, flow cytometry could be used as a rapid method for selection of optimal therapies, which may include drugs that target the Erk pathway. Here, we evaluated the utility of phospho-flow, compared to Western blotting, to monitor Erk pathway activation and its inhibition by targeted Mek kinase inhibitors in human BCP ALL… CONTINUE READING